1¡¢What is the overall design? The phosphorylation profiles were generated from four conditions of mice brain tissues; normal at the age of 2month, AD at the age of 2month, normal at the age of 6month and AD at the age of 6month. For each conditions, the profiling was done with three biological replicates (n=3). 2¡¢What is result of the experiment? To demonstrate the utility of the newly developed dendron-coated phosphokinase antibody array(DPA) in which the antibodies are immobilized on a dendron-coated glass slide, the phosphorylation profiles of brain tissue samples obtained from Alzheimer's disease (AD) model mice were generated. 3¡¢Your E-mail£º jeongdo801@postech.ac.kr e.g . 1¡¢What is the overall design? We used a proteome microarray containing ¡«4000 affinity-purified E. coli proteins to globally identify CobB interactors, and finally identified 183 binding proteins of high stringency. Bioinformatics analysis showed that these interacting proteins play a variety of roles in a wide range of cellular functions and are highly enriched in carboxylic acid metabolic process and hexose catabolic process, and also enriched in transferase and hydrolase. We further used bio-layer interferometry to analyze the interaction and quantify the kinetic parameters of putative CobB interactors, and clearly showed that CobB could strongly interact with TopA and AccC. 2¡¢What is result of the experiment? We identified 183 binding proteins of high stringency. 3¡¢Your E-mail£º microarray @protein--microarray.com