1¡¢What is the overall design? In this study, 18 tissue samples from bladder cancer or normal bladder mucosa were analyzed by protein antlbody array to investigate biomarker for bladder cancer. After the tissue samples were obtained intraoperatively, they were immediately frozen in liquid nitrogen and expressions of 656 proteins were analyzed using antibody microarray assay kit of Fullmoon Biosystems 2¡¢What is result of the experiment? 3¡¢Your E-mail£º imslee@gmail.com e.g . 1¡¢What is the overall design? We used a proteome microarray containing ¡«4000 affinity-purified E. coli proteins to globally identify CobB interactors, and finally identified 183 binding proteins of high stringency. Bioinformatics analysis showed that these interacting proteins play a variety of roles in a wide range of cellular functions and are highly enriched in carboxylic acid metabolic process and hexose catabolic process, and also enriched in transferase and hydrolase. We further used bio-layer interferometry to analyze the interaction and quantify the kinetic parameters of putative CobB interactors, and clearly showed that CobB could strongly interact with TopA and AccC. 2¡¢What is result of the experiment? We identified 183 binding proteins of high stringency. 3¡¢Your E-mail£º microarray @protein--microarray.com