| Accession |
14 |
| Title |
Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions |
| Summary |
Here we describe PCAs based on the enzyme TEM-1 beta-lactamase (EC: 3.5.2.6), which include simple colorimetric in vitro assays using the cephalosporin nitrocefin and assays in intact cells using the fluorescent substrate CCF2/AM (ref. 6). The combination of in vitro colorimetric and in vivo fluorescence assays of beta-lactamase in mammalian cells suggests a wide variety of sensitive and high-throughput large-scale applications, including in vitro protein array analysis of protein protein or enzyme protein interactions and in vivo applications such as clonal selection for cells expressing interacting protein partners. |
| Journal |
Nat Biotechnol. |
| Publish date |
June,2002 |
| Corresponding Author |
Stephen W. Michnick |
| E-mail |
stephen.michnick@umontreal.ca |
| Array Type |
Technological development of protein array |
| Species |
|
| Raw data link |
No relevant experiment link!
|
| Link to PubMed |
PubMed ID:12042868
|
