Accession 370
Title Unbiased discovery of interactions at a control locus driving expression of the cancer-specific therapeutic and diagnostic target, mesothelin
Summary We used two orthogonal proteomics approaches--unbiased SILAC (stable isotope labeling by amino acids in cell culture)/DNA affinity-capture/mass spectrometry survey (SD-MS) and a large transcription factor protein microarray (TFM)--and functional validation to explore systematically the CanScript interactome. SD-MS produced nine candidates, and TFM, 18. The screens agreed in confirming binding by TEAD proteins and by newly identified NAB1 and NFATc.
Journal J Proteome Res.
Publish date October, 2012
Corresponding Author Scott E. Kern; Akhilesh Pandey
E-mail skern1@jhmi.edu; pandey@jhmi.edu
Array Type Proteome array
Species Homo sapiens
Raw data link No relevant experiment link!
Link to PubMed

PubMed ID:23025254