| Accession |
370 |
| Title |
Unbiased discovery of interactions at a control locus driving expression of the cancer-specific therapeutic and diagnostic target, mesothelin |
| Summary |
We used two orthogonal proteomics approaches--unbiased SILAC (stable isotope labeling by amino acids in cell culture)/DNA affinity-capture/mass spectrometry survey (SD-MS) and a large transcription factor protein microarray (TFM)--and functional validation to explore systematically the CanScript interactome. SD-MS produced nine candidates, and TFM, 18. The screens agreed in confirming binding by TEAD proteins and by newly identified NAB1 and NFATc. |
| Journal |
J Proteome Res. |
| Publish date |
October, 2012 |
| Corresponding Author |
Scott E. Kern; Akhilesh Pandey |
| E-mail |
skern1@jhmi.edu; pandey@jhmi.edu |
| Array Type |
Proteome array |
| Species |
Homo sapiens |
| Raw data link |
No relevant experiment link!
|
| Link to PubMed |
PubMed ID:23025254
|
