Accession PMDE262
Name Serelogical autoantibody profiling of high myopia detected by HuProt array
Description Serum samples were probed using HuProt v3.1 arrays (CDI Laboratories, Inc.), which comprised of more than 20,000 unique human full-length proteins, according to the manufacturer's instruction. Briefly, the HuProt array is based on the sandwich immunoassay principle. More than 20,000 unique human proteins were duplicately immobilized in specific location spots on the surface of the array membrane. After blocking, HuProt array was incubated in 3 mL 1:1,000 diluted serum sample to capture corresponding autoantibodies to each protein spot, and followed with 1:2,000 diluted Cy3-conjugated goat anti-human IgG (Jackson, 109-165-008) and Alexa Fluor 647-affinipure donkey anti-human IgM (Jackson, 709-605-073) to fluorescently label IgG and IgM of captured autoantibodies, respectively. Finally, the HuProt assays were scanned using the GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, CA, USA) and analyzed with GenePix Pro 6.0 software (Molecular Devices, Sunnyvale, CA, USA).
Publication No relevant papers!
Provider Jiao Qi
Species Borrelia burgdorferi
Subspecies Empty
Array type Antibody array
Sample Serum
Number of array 36
Array(1) HuProt™20K
Raw data download
Experiment details download
Analysis report No relevant analysis report. If you want to analyse this data, please contact us.
Link to GEO No relevant GEO link!